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If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. The product is provided 'AS IS' and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

This product is intended for laboratory research use only.

A 5% CO 2 in air atmosphere is recommended if using the medium described on this product.

Incubate the culture at 37☌ in a suitable incubator.

It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). It is important to avoid excessive alkalinity of the medium during recovery of the cells.

Resuspend the cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm 2 or a 75 cm 2 culture flask.

Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 10 minutes.

All of the operations from this point on should be carried out under strict aseptic conditions.

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol.

Thawing should be rapid (approximately 2 minutes). To reduce the possibility of contamination, keep the O-ring and cap out of the water.

Thaw the vial by gentle agitation in a 37☌ water bath.

Storage at -70☌ will result in loss of viability. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70☌.

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt.